LaboratoryHPLC and GC Operation and Method Troubleshooting
High performance liquid chromatography and gas chromatography remain the backbone of pharmaceutical, environmental, and food safety testing, yet many operators are trained only to run methods, not to diagnose them when results drift. This hands on course covers column selection, mobile and stationary phase chemistry, and a systematic approach to chromatogram troubleshooting. Participants work through peak shape problems, retention time shifts, and baseline noise using real instrument data.
Course objectives
- Explain the separation principles underlying reversed phase HPLC and capillary GC
- Select appropriate columns, mobile phases, and temperature programs for a given separation
- Operate HPLC and GC systems including injection, detection, and integration settings
- Diagnose common chromatographic problems including tailing, splitting, and ghost peaks
- Apply system suitability testing to confirm method performance before reporting results
Who should attend
- Chromatography analysts in pharmaceutical and environmental laboratories
- Instrument operators transitioning from wet chemistry to chromatography
- Method development and validation scientists
- Laboratory supervisors overseeing chromatographic testing
Course outline
- 01Principles of separation in HPLC and GC
- 02Column chemistry, mobile phases, and stationary phases
- 03Instrument setup, injection technique, and detector selection
- 04System suitability testing and data integration
- 05Systematic troubleshooting of chromatographic faults
- 06Preventive maintenance and column care
Scheduled sessions
| Dates | Venue | Format | Price | Register |
|---|---|---|---|---|
| 16 to 18 October 2026 | Cape Town, South Africa | Classroom | R11,995 per delegate | Register Now |
| 8 to 10 November 2026 | Pretoria, South Africa | Classroom | R11,995 per delegate | Register Now |
| 26 to 28 March 2027 | Dubai, UAE | Classroom | R11,995 per delegate | Register Now |